Products/Services Used | Details | Operation |
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Peptide Library Services> | The HIV peptide pools used for intracellular cytokine staining were obtained from the AIDS Reagent Resource, and all other peptides were from AnaSpec or GenScript. MHC class I tetramers were obtained from the National Institutes of Health (NIH) tetramer facility at Emory University. | Get A Quote |
Type I interferons (IFN-I) are a major antiviral defense and are critical for the activation of the adaptive immune system. However, early viral clearance by IFN-I could limit antigen availability, which could in turn impinge upon the priming of the adaptive immune system. In this study, we hypothesized that transient IFN-I blockade could increase antigen presentation after acute viral infection. To test this hypothesis, we infected mice with viruses coadministered with a single dose of IFN-I receptor-blocking antibody to induce a short-term blockade of the IFN-I pathway. This resulted in a transient "spike" in antigen levels, followed by rapid antigen clearance. Interestingly, short-term IFN-I blockade after c... More